PROTOCOLS IN LIVESTOCK GENOME ANALYSIS

Cells are lysed using a detergent, and the lysate is alcohol precipitated.

The precipitates are removed by centrifugation, and the DNA is recovered by alcohol precipitation. Using a cesium chloride (CsCl) density gradient, genomic DNA can be purified by centrifugation

alting-out is a conventional technique where proteins and other

Silica Resin-Based Strategies, anion exchange Based Strategies, and Cesium chloride density gradients.

protocols for isolation of genomic DNA

For the purpose of DNA isolation, Leukocytes are the cells we are interested in. T

only blood cells that contain nuclei are leukocytes

components) do not contain nuclei and are therefore unsuitable for preparation of genomic DNA.

erythrocytes and thrombocytes (platelets, 0.5% of blood com

About 56% of blood volume is comprised of cells, more than 99% of which are erythrocytes. Human and livestock species